Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens)
نویسندگان
چکیده
To perform whole genome scanning for complex trait analysis, we isolated and characterized a total of 21 novel genomic-SSRs and EST-SSRs for yellow perch (Perca flavescens), using the methods of construction of SSR-enrichment libraries and EST database mining of a related species P. fluviatilis. Of 16 genomic-SSR primer pairs examined, eight successfully amplified scorable products. The number of alleles at these informative loci varied from 3 - 14 with an average of 8.5 alleles per locus. When tested on wild perch from a population in Pennsylvania, observed and expected heterozygosities ranged from 0.07 - 0.81 and from 0.37 - 0.95, respectively. Of 2,226 EST sequences examined, only 110 (4.93%) contained microsatellites and for those, 13 markers were tested, 12 of which exhibited polymorphism. Compared with genomic-SSRs, EST-SSRs exhibited a lower level of genetic variability with the number of alleles of averaging only 2.6 alleles per locus. Cross-species utility indicated that three of the genomic-SSRs and eight of the EST-SSRs successfully cross-amplified in a related species, the walleye (Sander vitreus).
منابع مشابه
Waterscape genetics of the yellow perch (Perca flavescens): patterns across large connected ecosystems and isolated relict populations.
Comparisons of a species' genetic diversity and divergence patterns across large connected populations vs. isolated relict areas provide important data for understanding potential response to global warming, habitat alterations and other perturbations. Aquatic taxa offer ideal case studies for interpreting these patterns, because their dispersal and gene flow often are constrained through narro...
متن کاملThe landscape genetics of yellow perch (Perca flavescens) in a large fluvial ecosystem.
Landscape genetics is being increasingly applied to elucidate the role of environmental features on the population structure of terrestrial organisms. However, the potential of this framework has been little explored in aquatic ecosystems such as large rivers. Here, we used a landscape genetics approach in order to (i) document the population structure of the yellow perch (Perca flavescens) by ...
متن کاملOn-farm tests show higher production, growth rate and survival of improved yellow perch
in the Proceedings of the World Aquaculture Society Conference. Page 1142. February 22-25, 2013. Nashville. Yellow perch Perca flavescens is a particularly important aquacultural and ecological species in the Great Lakes Region (GLR)and the Midwest USA. The demand for this species has remained very high in the GLR. Due to the relatively slow growth of currently cultured populations of yellow pe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- International Journal of Molecular Sciences
دوره 10 شماره
صفحات -
تاریخ انتشار 2009